High Quality Microquantity cDNA Libraries

High Quality Microquantity cDNA Libraries are constructed using our state-of-the-art methods for cDNA synthesis and cloning to produce libraries from small amounts of starting material. Like our standard libraries, these libraries are directionally cloned into Express I and then transformed into T1 phage resistant E. coli. We guarantee at least 1x10⁶ primary clones with 87% recombinant clones containing an average insert size of at least 1kb, however, we generally produce libraries of 3x10⁶ primary clones with >95% recombinant clones containing 1.5kb average insert size.

We will make microquantity libraries from customer supplied tissue (<1g), cells (at least 1x10⁷), total RNA (50 µg) or mRNA (500 ng). We provide quality controlled primary or amplified libraries.

Features list:

- High quality cDNA clones.
- Directionally cloned cDNA for expression, antibody screening, subtraction and normalization.
- Large numbers of primary clones.
- Low vector background.

Quality testing: Percentage of recombinant clones and average insert size determined by gel analysis of 24 clones picked at random.

Shipment and storage conditions: Libraries will be shipped in dry ice. Storage of libraries at -80°C is recommended.

*Average insert size and insert size range determined by restriction enzyme digestion of 24 clones picked at random from each library.