High Quality Large Insert cDNA Libraries are constructed using our state-of-the-art methods for cDNA synthesis and size fractionation to produce libraries containing the largest cDNA inserts available. Although these libraries are made using a different process, they share the same advantageous features as our standard libraries (directional cloning into Express I and T1 phage resistant E. coli). We guarantee at least 1x10⁶ primary clones with 87% recombinant clones containing an average insert size of at least 3kb, however, we generally produce libraries of 3x10⁶ primary clones with >95% recombinant clones containing 4kb average insert size.

We will make large insert libraries from customer supplied tissue (2 g), cells (at least 2x10⁸), total RNA (2 mg) or mRNA (10 µg). We provide quality controlled primary or amplified libraries.

Features list:

- High quality size fractionated cDNA.
- Directionally cloned cDNA for expression, antibody screening, subtraction and normalization.
- Large numbers of primary clones.
- Low vector background.

Quality testing: Percentage of recombinant clones and average insert size determined by gel analysis of 24 clones picked at random.

Shipment and storage conditions: Libraries will be shipped in dry ice. Storage of libraries at -80°C is recommended.

*Average insert size and insert size range determined by restriction enzyme digestion of 24 clones picked at random from each library.