cDNA Nanoquantity Library Data
*Average insert size and insert size range determined by restriction
Characteristics of a cDNA Library Constructed From a Nanoquantity
Amount of HeLa mRNA
mRNA (ng)
Total # of primary clones
Average insert size (kb)*
Percent recombinants
Insert size range (kb)*
100
7.2x107
1.20
100
0.5 - 3.2
50
1.5x107
1.35
100
0.6 - 3.5
10
1.7x106
1.00
100
0.4 - 2.9
5
9.0x105
1.01
96
0.4 - 3.0
2
2.0x105
0.74
94
0.2 - 2.3
1
4.5x104
0.92
94
0.2 - 3.6
enzyme digestion of 24 clones picked at random from each library.