cDNA Microquantity Library Data
*Average insert size and insert size range determined by restriction
Characteristics of a cDNA Library Constructed From a Microquantity
Amount of HeLa mRNA
mRNA (ng)
Total # of primary clones
Average insert size (kb)*
Percent recombinants
Insert size range (kb)*
2,000
7.3x107
2.43
100
1.7 - 5.5
1,000
8.0x107
2.43
100
0.9 - 5.2
500
6.7x107
1.75
100
0.8 - 2.9
enzyme digestion of 24 clones picked at random from each library.