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High Quality Standard cDNA Libraries
High Quality Standard cDNA Libraries are produced using our proprietary
technology. First strand cDNA is made from mRNA that is primed using oligo
dT. After the second strand is synthesized, the double stranded cDNA is size
fractionated, cloned directionally into our Express I vector and transformed
into T1 phage resistant E. coli. We guarantee at least 3x106
primary clones with
³87% recombinant clones containing
an average insert size of at least 1kb, however, we generally produce libraries
of 10x106 primary clones with >95% recombinant clones containing
³1.5kb average insert size.
We will make standard libraries from customer supplied tissue (³1 g), cells (at least 1x108), total RNA (³1 mg) or mRNA (³5 µg). We provide
quality controlled primary or amplified libraries.
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