High Quality Nanoquantity cDNA Libraries

Recently, we developed methods that enable us to produce High Quality Nanoquantity cDNA Libraries from such small amounts of tissue and cells that material prepared by laser capture microdissection (LCM) or FACS can be used. Like our standard libraries, these libraries are directionally cloned into Express I and then transformed into T1 phage resistant E. coli. We guarantee at least 2x105 primary clones with ³87% recombinant clones containing an average insert size of at least 800 bp, however, we generally produce libraries of 5x105 primary clones with >95% recombinant clones containing ³1.0kb average insert size.

We will make nanoquantity libraries from customer supplied tissue (at least 250 µg), cells (at least 25,000), total RNA (³250 ng) or mRNA (³5 ng). We provide quality controlled primary or amplified libraries.

 
Features list:
  • High quality cDNA clones.
  • Directionally cloned cDNA for expression, antibody screening, subtraction and normalization.
  • Large numbers of primary clones.
  • Low vector background.
 
Quality testing: Percentage of recombinant clones and average insert size determined by gel analysis of 24 clones picked at random.
 
Shipment and storage conditions: Libraries will be shipped in dry ice. Storage of libraries at -80°C is recommended.


Please click here to view data from our nanoquantity cDNA libraries
Please click here for our cDNA Library Construction Pricing Information

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