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High Quality Large Insert cDNA Libraries High Quality Large Insert cDNA Libraries are constructed using our state-of-the-art methods for cDNA synthesis and size fractionation to produce libraries containing the largest cDNA inserts available. Although these libraries are made using a different process, they share the same advantageous features as our standard libraries (directional cloning into Express I and T1 phage resistant E. coli). We guarantee at least 1x106 primary clones with ³87% recombinant clones containing an average insert size of at least 3kb, however, we generally produce libraries of 3x106 primary clones with >95% recombinant clones containing ³4kb average insert size. We will make large insert libraries from customer supplied tissue (³2 g), cells (at least 2x108), total RNA (³2 mg) or mRNA (³10 µg). We provide quality controlled primary or amplified libraries. |
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