cDNA Large Insert Library Data
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Characteristics of a cDNA Library Constructed From a Large Insert Amount of HeLa mRNA |
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| mRNA (ng) | Total # of primary clones | Average insert size (kb)* | Percent recombinants | Insert size range (kb)* |
| 5,000 | 2.1x107 | 4.26 | 96 | 1.0 - 6.2 |
| 2,000 | 2.5x107 | 4.46 | 100 | 2.4 - 7.2 |
*Average insert size and insert size range determined by restriction
enzyme digestion of 24 clones picked at random from each library.